With a little practice, you can scan a chromatogram in less than a minute and spot problems. Occasionally, information in this document will refer to older sequencers for historical purposes, and we will specifically state when this is the case. The current version of this document reflect our current generation of DNA sequencers, the ABI Model 3730XL. truncate the sequence when errors become too frequent.It is the responsibility of the Core clients to: Links are also given for more complete troubleshooting of problems. This document explains how to examine the normal DNA sequencing chromatogram, describing common issues and how to interpret them. Other errors can crop up in the middle, invalidating individual base calls or entire swaths of data. Predictable errors occur near the beginning and again at the end of any sequencing run. That computer program, however, does make mistakes and you need to manually double-check the interpretation of the primary data. Truncate the sequence where problems become too frequentĪutomated DNA Sequencers generate a four-color chromatogram showing the results of the sequencing run, as well as a computer program’s best guess at interpreting that data – a text file of sequence data.Get a general sense of how clean the sequence is.
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